| Users should fully read the instructions on ASCEM website, the following only highlights some frequently asked questions. |
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Case Application – Also open to academic and industry users off-campus |
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| Q1. How to book online? Ans. |
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| Q2. Which instrument should be selected? Ans. |
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| Q3. How to apply an instrument? Ans. |
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| Q4. Which instrument should be used for Liposome/Exoseome/Polymer samples? Ans. |
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Q5. Taking CEM2 as an example, how long does it approximately take for a sample from cryo-grids preparation to
experiment completion? Ans. |
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| Q6. How many hours that need to be booked for conducting an experiment on CEM1? Ans. |
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| Q7. Need ASCEM’s feedback on whether a protein sample is suitable for cryo-EM structural studies? Ans. |
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Sample Preparation and data collection Ans. |
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| Q1. What are the basic requirements for protein samples? (very important) |
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| Q2. What quality control methods can be taken before the protein sample submission? (not requisite,
but very helpful) |
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| Q3. What are the pre-treatments or buffer restrictions for samples doing the negative staining? |
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Q4. Taking liposomes as the example, what should be processed or paid attention to before delivering samples to
ASCEM? |
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| Q5. What solvent should be used for cryo-grid sample preparation? |
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| Q6. Do Users need to prepare consumables or standards? |
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| Q7. What type of grids should be used for Cryo-EM? Does ASCEM provide the grids? |
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| Q8. Does ASCEM provide original Vitrobot filter papers? |
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| Q9. Can multiple people be on site together when using instrument? |
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| Q10. Is Wifi available in the lab? |
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| Q1. How to pay for the service? Ans. |
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| Q2. Taking CEM2 application as an example, how to charge for a single experiment? Ans. |
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Q3. Taking CEM2 as an example, if the experiment actually took one hour only, will it still be charged as one session
(3 hours)? Ans. |
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Facility Reservation System |
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| Q1. What are the procedures for applying for facility services via the DAAIS Reservation System? Ans. |
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Q2. What are the other ways to process “case application” and “authority application” if the LINK auto-sent by the
DAAIS Reservation System was missed? Ans. |
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| Q3. Why can’t PI sometimes find the records of “case application”? Ans. |
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| Q4. What to do if a registered user needs to change PI and institute due to position change or resignation? Ans. |
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| Q5. What should be done when a user needs to update his or her identity as a PI? Ans. |
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