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Usage Overview |
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Appliances |
CEM1
JEOL-JEM1400 |
Vitrobot |
CEM2
TF20 |
CEM3
Talos Arctica |
CEM4
Titan Krios |
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Experiment Category |
Negative Stain /
Rm. Temp. TEM |
Sample
Preparation |
Cryo-EM
(Negative Stain) |
Cryo-EM
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Cryo-EM
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| Single Particle Analysis |
| Liposome / Polymer etc. |
| Cryo-tomography |
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*The instruments of CLEM for cryo-fluorescence and of FIB for milling are external equipment that are respectively managed
by the Institute of Plant and Microbial Biology (IPMB) and the Research Center for Applied Sciences (RCAS), Academia
Sinica. Because they are not open to users outside the institutes, and must be equipped with special accessories for
biological experiments, please contact ASCEM first if necessary.
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Transmission Electron Microscopes (TEM) |
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CEM1 - JEOL JEM-1400 |
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Room temperature TEM. Max. 120kV; Gatan CCD camera. Easy for operation, high quality images, can work as a cryo-EM as well. |
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Mainly for negative staining, the first step of checking the quality of protein or particle samples in the total cryo-EM workflow. Applicable to low-contrast sample observation in medicine, biology, clinics, immunology, pharmaceuticals, food, polymers etc. |
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Location |
TEM room, 1st floor, Library of Institute of Chemistry |
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Contact |
Dr. Wei-Hau Chang / Ms. Hui-Ju Huang / Ms. Yu-Hua Chang |
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CEM2 - FEI Tecnai G2 F20 TWIN |
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Max. 200k; Gatan 4K X 4K CCD and DE20 high-sensitivity camera. |
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Mainly for biological microstructure analysis, preliminary study of cryo-EM or cryo-tomography, sample screening and a low-resolution model building. |
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Also supports other studies, such as liposome and polymer etc. |
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Location |
Rm. A102, B2, Interdisciplinary Research Building of Sci. & Tech. |
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Contact |
Dr. Yuan-Chih Chang / Ms. Hui-Ju Huang |
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CEM3 - FEI Talos Arctica 200 keV |
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Falcon 3EC detector; Volta phase plates; multi-purpose. |
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Mainly for automated condition screening and publication quality studies of both cryo-EM and cryo-TM, including overflow of the preliminary study samples from CEM2, screening of grids for CEM4, data collection of high quality data for structural determination (up to 4 angstrom or a little better) and publication. |
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Location |
Rm. A9, B2, Interdisciplinary Research Building of Sci. & Tech. |
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Contact |
Dr. Irene Deli / Ms. Yu-Hua Chang |
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CEM4 - FEI Titan Krios 300 keV |
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Falcon 3EC and K3 detectors; Volta phase plates. |
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Mainly for data collection for high-resolution structures. Preliminary sample, with biological significance, checking by CEM1/2/3 is recommended. |
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Location |
Rm. A8, B2, Interdisciplinary Research Building of Sci. & Tech. |
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Contact |
Dr. Chun-Hsiung Wang |
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Instruments for sample preparation |
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| Location |
Rm. A8, B2, Interdisciplinary Research Building of Science and Technology |
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| Contact |
Dr. Yuan-Chih Chang / Ms. Hui-Ju Huang |
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Gatan Solarus 950 Plasma Cleaning System |
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Model 950. Superior cleaning recipes and ratio of H2/O2/Ar. |
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Mainly for TEM samples, e.g. holey carbon grids, cleaning, remove hydrocarbon contamination. Supports grids preparation for SPA and cryo-tomography experiments. |
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PELCO easiGlow 91000 Glow Discharge System |
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Model 91000. Dual chamber to support hydrophilic and hydrophobic surface treatments separately to avoid cross contamination of chambers. |
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Mainly for TEM grids cleaning and carbon support films hydrophilic surface treatments. Supports grids preparation for SPA and cryo-tomography experiments. |
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Jelight UVO-cleaner |
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MODEL 18. |
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Mainly for cryo-grids cleaning prior to CEM3/4 data collection, removing organic contaminants. |
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FEI Vitrobot |
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Type IV。Instant cool bio-samples to the temperature of liquid nitrogen in less than microseconds, and freeze bio-molecules in very thin amorphous ice. |
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Mainly for SPA and cryo-Tomography experiments. Also supports other studies such as liposome and polymer, etc. |
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Computing System |
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ASCEM cooperates with the Core Facility of Big Data Analysis & Scientific Computing Academia Sinica (formerly ASGC) to construct a user-based integrated system of data transfer and process to cope with the huge amount of data of cryo-EM experiments. Services provided by ASGC will follow ASGC regulations, including charging schemes.
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