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Usage Overview |
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Appliances |
Vitrobot |
CEM2
TF20 |
CEM3
Talos Arctica |
CEM4
Titan Krios |
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Experiment Category |
Sample
Preparation |
Cryo-EM
(Negative Stain) |
Cryo-EM
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Cryo-EM
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| Single Particle Analysis |
| Liposome / Polymer etc. |
| Cryo-tomography |
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Transmission Electron Microscopes (TEM) |
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CEM2 - FEI Tecnai G2 F20 TWIN |
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Max. 200k; Gatan 4K X 4K CCD and DE20 high-sensitivity camera. |
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Mainly for biological microstructure analysis, preliminary study of cryo-EM or cryo-tomography, sample screening and a low-resolution model building. |
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Also supports other studies, such as liposome and polymer etc. |
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Location |
Rm. A10, B2, Interdisciplinary Research Building of Sci. & Tech. |
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Contact |
Dr. Yuan-Chih Chang / Ms. Hui-Ju Huang |
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CEM3 - FEI Talos Arctica 200 keV |
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Falcon 3EC detector; Volta phase plates; multi-purpose. |
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Mainly for automated condition screening and publication quality studies of both cryo-EM and cryo-TM, including overflow of the preliminary study samples from CEM2, screening of grids for CEM4, data collection of high quality data for structural determination (up to 4 angstrom or a little better) and publication. |
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Location |
Rm. A9, B2, Interdisciplinary Research Building of Sci. & Tech. |
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Contact |
Dr. Yen-Song Chen / Ms. Yu-Hua Chang |
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CEM4 - FEI Titan Krios 300 keV |
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Falcon 3EC and K3 detectors; Volta phase plates. |
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Mainly for data collection for high-resolution structures. Preliminary sample, with biological significance, checking by CEM1/2/3 is recommended. |
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Location |
Rm. A8, B2, Interdisciplinary Research Building of Sci. & Tech. |
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Contact |
Dr. Chun-Hsiung Wang |
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Instruments for sample preparation |
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| Location |
Rm. A11, B2, Interdisciplinary Research Building of Science and Technology |
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| Contact |
Dr. Yuan-Chih Chang / Ms. Hui-Ju Huang |
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Gatan Solarus 950 Plasma Cleaning System |
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Model 950. Superior cleaning recipes and ratio of H2/O2/Ar. |
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Mainly for TEM samples, e.g. holey carbon grids, cleaning, remove hydrocarbon contamination. Supports grids preparation for SPA and cryo-tomography experiments. |
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PELCO easiGlow 91000 Glow Discharge System |
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Model 91000. Dual chamber to support hydrophilic and hydrophobic surface treatments separately to avoid cross contamination of chambers. |
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Mainly for TEM grids cleaning and carbon support films hydrophilic surface treatments. Supports grids preparation for SPA and cryo-tomography experiments. |
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Jelight UVO-cleaner |
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MODEL 18. |
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Mainly for cryo-grids cleaning prior to CEM3/4 data collection, removing organic contaminants. |
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FEI Vitrobot |
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Type IV。Instant cool bio-samples to the temperature of liquid nitrogen in less than microseconds, and freeze bio-molecules in very thin amorphous ice. |
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Mainly for SPA and cryo-Tomography experiments. Also supports other studies such as liposome and polymer, etc. |
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Computing System |
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ASCEM cooperates with the Core Facility of Big Data Analysis & Scientific Computing Academia Sinica (formerly ASGC) to construct a user-based integrated system of data transfer and process to cope with the huge amount of data of cryo-EM experiments. Services provided by ASGC will follow ASGC regulations, including charging schemes.
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